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KMID : 0368419790220040095
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Journal of Plant Biology
1979 Volume.22 No. 4 p.95 ~ p.100
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Studies on the Mechanisms of Gibberellic Acid Action
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Abstract
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As a part of the studies on the regulatory mechanism of gene expression by GA_3, the effects of GA_3 on the protein biosynthesis and phosphorylation in maize seedlings were investigated.
1. The optimum concentration of GA_3 for the stimulation of the protein biosynthesis was 0.3mM.
2. The protein biosynthesis was remarkably increase by GA_3 during the germination. The reason for the decrease in the protein biosynthesis by 48hrs. after germination seems to be a staggered gene expression, and/or increases in protease and RNase activities.
3. The ratio of the amount of the newly synthesized protein in germination seeds treated with GA_3 to the amount of proteins secreted into the endosperm was similar to that ratio in control. According to this result, it seems that GA_3 stimulates only the expression of certain definite genes.
4. By the treatment with GA_3, the rates of biosynthesis and phosphorylation of proteins were increased up to about 1.5 times during germination and 6 times by 72hrs. after germination, respectively. The ratio of the total soluble proteins to the phosphoproteins considerably increased in teh early germination stage (24hrs.) but decreased after 24hrs. According to the above mentioned results, the stimulation of the phosphorylation of proteins by GA_3 seems to be attributed to the increases in the activities of protein kinases.
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